Coding
Part:BBa_K4481000:Design
Designed by: Anne Byford Group: iGEM22_GastonDay-Shangde (2022-09-30)
smPAL codon optimized for E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 72
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The published sequence was altered to remove restriction sites without changing the ultimate amino acid sequence and then optimized for E. coli.
Source
Part was produced by IDT from a sequence published by Bang, et al (2016) paper published in Microbial Cell Factories.