Coding

Part:BBa_K4481000:Design

Designed by: Anne Byford   Group: iGEM22_GastonDay-Shangde   (2022-09-30)


smPAL codon optimized for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 72
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The published sequence was altered to remove restriction sites without changing the ultimate amino acid sequence and then optimized for E. coli.


Source

Part was produced by IDT from a sequence published by Bang, et al (2016) paper published in Microbial Cell Factories.

References